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Biblioteca(s):  Epagri-Sede.
Data corrente:  23/08/2013
Data da última atualização:  23/08/2013
Tipo da produção científica:  Artigo em Periódico Indexado
Autoria:  VESCO, L. L. D.; PINTO,; ZAFFARI, G. R.; NODARI, R. O.; GUERRA, M. P.
Título:  Improving pineapple micropropagation protocol through explant size and medium composition manipulation.
Ano de publicação:  2001
Fonte/Imprenta:  Fruits, Paris, v. 56, n. 3, p. 143-154, 2001.
Idioma:  Português
Conteúdo:  Abstract - Introduction. Although several pineapple micropropagation ProtocoIs have aIready been published, signifieant improvement eouId be achieved if the stages of the in vitro eulture were better defined. Our work coneemed several experiments aiming at the mass produetion of high quality plantlets. MateriaIs and metbods. Axillary buds were inoculated on an MS líquíd eulture medium added With NAA (2/lM) and BAP (4 /lM). Regenerated shoots, divided into three classes of different sízas, were then used In further experiments. First, these shoots were inocuIated in flasks eontaining the same MS cultura medium With or Without growth regulators. Then, four basíc media, eontaining different salts and free from growrh reguIators were tested. In a third assay, the MS eulture medium was eompared wíth a half-diluted MS culture medium for studying theplantlet elongation and rootíng stage. Results. In the MS euIture medium supplemented With NAA and BAP, the highest muItiplication rate (13.5 shoots) was obtained wíth the smallest shoots inocuIated, whíle in the MS eulture medium free Df growth regulators, the highest plantlets (7.7 em) were the result of the highest shoots inoculated and showed no vitrification. The normal MS cuIture medium, in eomparison With the half-diluted one and the three other saIt fonnuIations, revealed a signifleant increase in the plantlet elongation and best general features. For accIimatization, the highest values of the survival rate (93.8%) and fresh and... Mostrar Tudo
Palavras-Chave:  Ananas comosus; Brazil; Culture media; In vitro experimentation; Plant propagation.
Categoria do assunto:  F Plantas e Produtos de Origem Vegetal
Marc:  Mostrar Marc Completo
Registro original:  Epagri-Sede (Epagri-Sede)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status
Epagri-Sede94015 - 1UPCAP - DD


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