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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
23/08/2013 |
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Data da última atualização: |
23/08/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VESCO, L. L. D.; PINTO,; ZAFFARI, G. R.; NODARI, R. O.; GUERRA, M. P. |
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Título: |
Improving pineapple micropropagation protocol through explant size and medium composition manipulation. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Fruits, Paris, v. 56, n. 3, p. 143-154, 2001. |
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Idioma: |
Português |
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Conteúdo: |
Abstract - Introduction. Although several pineapple micropropagation ProtocoIs have aIready been published, signifieant improvement eouId be achieved if the stages of the in vitro eulture were better defined. Our work coneemed several experiments aiming at the mass produetion of high quality plantlets. MateriaIs and metbods. Axillary buds were inoculated on an MS líquíd eulture medium added With NAA (2/lM) and BAP (4 /lM). Regenerated shoots, divided into three classes of different sízas, were then used In further experiments. First, these shoots were inocuIated in flasks eontaining the same MS cultura medium With or Without growth regulators. Then, four basíc media, eontaining different salts and free from growrh reguIators were tested. In a third assay, the MS eulture medium was eompared wíth a half-diluted MS culture medium for studying theplantlet elongation and rootíng stage. Results. In the MS euIture medium supplemented With NAA and BAP, the highest muItiplication rate (13.5 shoots) was obtained wíth the smallest shoots inocuIated, whíle in the MS eulture medium free Df growth regulators, the highest plantlets (7.7 em) were the result of the highest shoots inoculated and showed no vitrification. The normal MS cuIture medium, in eomparison With the half-diluted one and the three other saIt fonnuIations, revealed a signifleant increase in the plantlet elongation and best general features. For accIimatization, the highest values of the survival rate (93.8%) and fresh and dry weights were obtained With the transferenee of higher than 7.0 em in vitro plantlets. Conclusion. Using the Protocol deseribed in this work, it is possíbls to obtaín 1 million in vitro plantlets after 9 months from a síngls bud, With a 45 day subculture interval and an average multiplieation rate of 10 shoots per bud. MenosAbstract - Introduction. Although several pineapple micropropagation ProtocoIs have aIready been published, signifieant improvement eouId be achieved if the stages of the in vitro eulture were better defined. Our work coneemed several experiments aiming at the mass produetion of high quality plantlets. MateriaIs and metbods. Axillary buds were inoculated on an MS líquíd eulture medium added With NAA (2/lM) and BAP (4 /lM). Regenerated shoots, divided into three classes of different sízas, were then used In further experiments. First, these shoots were inocuIated in flasks eontaining the same MS cultura medium With or Without growth regulators. Then, four basíc media, eontaining different salts and free from growrh reguIators were tested. In a third assay, the MS eulture medium was eompared wíth a half-diluted MS culture medium for studying theplantlet elongation and rootíng stage. Results. In the MS euIture medium supplemented With NAA and BAP, the highest muItiplication rate (13.5 shoots) was obtained wíth the smallest shoots inocuIated, whíle in the MS eulture medium free Df growth regulators, the highest plantlets (7.7 em) were the result of the highest shoots inoculated and showed no vitrification. The normal MS cuIture medium, in eomparison With the half-diluted one and the three other saIt fonnuIations, revealed a signifleant increase in the plantlet elongation and best general features. For accIimatization, the highest values of the survival rate (93.8%) and fresh and... Mostrar Tudo |
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Palavras-Chave: |
Ananas comosus; Brazil; Culture media; In vitro experimentation; Plant propagation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02504naa a2200229 a 4500
001 1118894
005 2013-08-23
008 2001 bl uuuu u00u1 u #d
100 1 $aVESCO, L. L. D.
245 $aImproving pineapple micropropagation protocol through explant size and medium composition manipulation.$h[electronic resource]
260 $c2001
520 $aAbstract - Introduction. Although several pineapple micropropagation ProtocoIs have aIready been published, signifieant improvement eouId be achieved if the stages of the in vitro eulture were better defined. Our work coneemed several experiments aiming at the mass produetion of high quality plantlets. MateriaIs and metbods. Axillary buds were inoculated on an MS líquíd eulture medium added With NAA (2/lM) and BAP (4 /lM). Regenerated shoots, divided into three classes of different sízas, were then used In further experiments. First, these shoots were inocuIated in flasks eontaining the same MS cultura medium With or Without growth regulators. Then, four basíc media, eontaining different salts and free from growrh reguIators were tested. In a third assay, the MS eulture medium was eompared wíth a half-diluted MS culture medium for studying theplantlet elongation and rootíng stage. Results. In the MS euIture medium supplemented With NAA and BAP, the highest muItiplication rate (13.5 shoots) was obtained wíth the smallest shoots inocuIated, whíle in the MS eulture medium free Df growth regulators, the highest plantlets (7.7 em) were the result of the highest shoots inoculated and showed no vitrification. The normal MS cuIture medium, in eomparison With the half-diluted one and the three other saIt fonnuIations, revealed a signifleant increase in the plantlet elongation and best general features. For accIimatization, the highest values of the survival rate (93.8%) and fresh and dry weights were obtained With the transferenee of higher than 7.0 em in vitro plantlets. Conclusion. Using the Protocol deseribed in this work, it is possíbls to obtaín 1 million in vitro plantlets after 9 months from a síngls bud, With a 45 day subculture interval and an average multiplieation rate of 10 shoots per bud.
653 $aAnanas comosus
653 $aBrazil
653 $aCulture media
653 $aIn vitro experimentation
653 $aPlant propagation
700 1 $aPINTO,
700 1 $aZAFFARI, G. R.
700 1 $aNODARI, R. O.
700 1 $aGUERRA, M. P.
773 $tFruits, Paris$gv. 56, n. 3, p. 143-154, 2001.
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